Cart
Products
Request a quote for a bulk order Request Quote
Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
PRMT2 (Protein Arginine Methyltransferase 2) is a member of the protein arginine N-methyltransferase (PRMT) family that is capable of monomethylating and asymmetrically dimethylating arginine residues. PRMT2 methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, and histone H4. PRMT2, along with NCOA2, acts as a coactivator of androgen receptor (AR)-mediated transactivation. PRMT2 also acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. PRMT2 enhances PGR, PPARϒ, RARA-mediated transactivation and may inhibit NFκB transcription and promote apoptosis. PRMT2 is known to repress E2F1 transcriptional activity in an Rb-dependent manner and may be involved in growth regulation.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant PRMT2 was expressed in a baculovirus expression system as the full length protein (accession number NP_996845.1) with an N-terminal FLAG-Tag. The molecular weight of PRMT2 is 47.6 kDa. The purity of the protein is >75% by SDS-PAGE.
Recombinant PRMT2 protein gel.
PRMT2 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
