Recombinant SMYD3 protein

Aliases: KMT3E; ZMYND1; ZNFN3A1; bA74P14.1
Catalog No: 31407 Format: 20 µg ¥88,000 Buy
Catalog No: 31807 Format: 1 mg ¥770,000 Buy

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

SET and MYND domain-containing protein 3 (SMYD3) is a member of the SMYD family of histone methyltransferases that specifically methylates lysine 4 of histone H3 (H3K4), inducing di- and tri-methylation, but not monomethylation. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex. It has also been shown to bind DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. SMYD3 expression has been shown to be upregulated in certain cancers.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant SMYD3 (accession number NP_001161212.1) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 50.7 kDa. The recombinant protein is >95% pure by SDS-PAGE.

 
SMYD3 Coomassie gel

Recombinant SMYD3 protein gel.
SMYD3 protein was run on an SDS-PAGE gel and stained with Coomassie Blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.