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JMJD2D antibody (pAb)
| RRID: AB_2793203 |
| Aliases: KDM4D |
| Catalog No: 39247 | Format: 100 µl | ¥86,000 | Buy |
| Catalog No: 39248 | Format: 10 µl | ¥24,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 1:500 - 1:2,000 dilution
Immunogen
This JMJD2D antibody was raised against a peptide corresponding to amino acids 337-359 of human JMJD2D.
Buffer
Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
JMJD2D pAb tested by Western blot.
Nuclear extract of HeLa cells (20 µg) probed with JMJD2D pAb (1:1,000). Specificity was verified by peptide competition.
Lane 1: No peptide.
Lane 2: 1 µM immunizing peptide.
Background
JMJD2D (KDM4D) is a histone demethylase located in the nucleus that specifically demethylates Lysine 9 of Histone H3, thereby playing a central role in the histone code. JMJD2D (KDM4D) demethylates both di- and trimethylated histone H3 Lys 9, while it has no activity on monomethylated residues. The demethylation reaction of the Lys residue generates formaldehyde and succinate as a by product. In addition, JMJD2D forms a complex with the ligand-bound form of the Androgen Receptor (AR) and, through this interaction, activates AR expression. Overexpression of AR is associated with prostate cancer, suggesting that, via its ability to upregulate AR, JMJD2D (KDM4D) may be involved in carcinogenesis.
Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for JMJD2D antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
