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AID antibody (mAb)
| RRID: AB_2793380 |
| Clone: 328.8b |
| Aliases: AICDA, ARP2, CDA2, HIGM2, HEL-S-284 |
| Catalog No: 39885 | Format: 100 µg | ¥88,000 | Buy |
| Catalog No: 39886 | Format: 10 µg | ¥24,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 1 - 3 µg/ml dilution
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This AID antbody was raised against a peptide corresponding to amino acids 178-198 of human AID.
Buffer
Purified IgG in 50 mM Tris-HCl (pH 7.2), 30% glycerol, and 0.035% sodium azide. Sodium azide is highly toxic.
AID antibody (mAb) tested by Western blot.
Murine spleen cells were probed with AID antibody at a dilution of 3 µg/ml.
Lane 1: Unstimulated spleen cells from wild type mice.
Lane 2: Treated (LPS & IL4 for 96 hrs) spleen cells from wild type mice.
Lane 3: Unstimulated spleen cells from AID knock-out mice.
Lane 4: Treated (LPS & IL4 for 96 hrs) spleen cells from AID knock-out mice.
Background
AID (Activation-induced cytidine deaminase, AICDA) is a member of the cytidine and deoxycytidylate deaminase family. It acts act as a single-strand DNA deaminase involved in somatic hypermutation, gene conversion and class-switch recombination in developing B-cells and is necessary for efficient antibody responses. It has also been implicated in active DNA demethylation, as deamination of methylcytosine yields thymine, which is read as a mismatch and is replaced by DNA damage repair enzymes.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AID antibody (mAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
