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SMC3 antibody (pAb)
| RRID: AB_2688005 |
| Aliases: BAM, BMH, HCAP, CDLS3, CSPG6, SMC3L1 |
| Catalog No: 61131 | Format: 100 µl | ¥86,000 | Buy |
| Catalog No: 61132 | Format: 10 µl | ¥24,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
WB*: 1:500 - 1:1,000 dilution
*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This SMC3 antibody was raised against a peptide in the C-terminal region of human SMC3.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
SMC3 antibody (pAb) tested by Western blot.
Detection of SMC3 by Western blot analysis. Lane 1: Nuclear extract of HeLa cells (20 µg). Lane 2: Nuclear extract of Hep G2 cells (20 µg). Lane 3: Nuclear extract of HEK293 cells (20 µg). Lane 4: Nuclear extract of U2OS cells (20 µg).
All were probed with SMC3 antibody (pAb) at a 1:500 dilution.
Background
The Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. SMC3 is a component of the cohesin complex that plays an essential role during chromatid segregation. SMC3 is also involved in DNA recombination and repair, mitotic chromosome organization and microtubule-mediated intracellular transport. SMC3 is acetylated during replication, which is essential for cohesion, and is phosphorylated by ATM subsequent to DNA double strand breakage. SMC3 is frequently elevated in human colon carcinoma and overexpression of the protein transforms fibroblasts.
Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for SMC3 antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
