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JMJD2B / KDM4B antibody (pAb)
| RRID: AB_2615033 |
| Aliases: TDRD14B |
| Catalog No: 61221 | Format: 100 µl | ¥86,000 | Buy |
| Catalog No: 61222 | Format: 10 µl | ¥24,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
ICC/IF: 1:2,000 dilution
IP: 10 µl per IP
WB*: 1:500 - 1:1,000 dilution
*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
For JMJD2B / KDM4B, we also offer AbFlex® JMJD2B / KDM4B Recombinant Antibody (rAb). For details, see Catalog No. 91305.
Immunogen
This JMJD2B / KDM4B antibody was raised against a peptide within the C-terminal region of human JMJD2B.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
JMJD2B / KDM4B antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with JMJD2B / KDM4B antibody at a 1:2,000 dilution.
JMJD2B / KDM4B antibody (pAb) tested by Immunoprecipitation.
4 µl of JMJD2B / KDM4B antibody was used to immunoprecipitate JMJD2B / KDM4B from 500 µg of K562 whole cell extract (lane 2). 4 µg of mouse IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the JMJD2B / KDM4B antibody at a dilution of 1:500.
JMJD2B / KDM4B antibody (pAb) tested by Western blot.
Nuclear extract of K-562 cells (40 µg) probed with JMJD2B antibody (pAb) at a dilution of 1:500.
Background
JMJD2B / KDM4B (JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, JHDM3B) is a histone demethylase that has been shown to demethylate histone H3 trimethylated at lysine 9. JMJD2B / KDM4B is physically associated with the MLL2 methyltransferase complex where it serves as a co-factor for ERα-mediated transcriptional activation.
Positive Control
Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for JMJD2B / KDM4B antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
