JunD antibody (pAb)

RRID: AB_2793621
Aliases: AP-1
Catalog No: 61403 Format: 100 µl ¥88,000 Buy
Catalog No: 61404 Format: 10 µl ¥24,000 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
40 & 45 kDa
Reactivity:
Human

Applications

CUT&RUN Validated ChIP Validated ChIP-Seq Validated Western Blot Validated Immunoprecipitation Validated

Application Notes

Applications Validated by Active Motif:
ChIP-Seq: 2 - 5 µl per ChIP
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution
CUT&RUN: 1 µl per 50 µl reaction

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-Seq

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This antibody was raised against a peptide within the N-terminal region of human JunD.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

JunD antibody (pAb) tested by ChIP-Seq
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from human endometrial stromal cell chromatin and 4 µg of JunD antibody. ChIP DNA was sequenced on the Illumina HiSeq and 8.6 million sequence tags were mapped to identify JunD binding sites on chromosome 16. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

JunD antibody (pAb) tested by Immunoprecipitation.
10 µl of JunD antibody was used to immunoprecipitate JunD from 250 µg of K562 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the JunD antibody at a dilution of 1:1,000.

JunD antibody (pAb) tested by Western blot.
K-562 nuclear extract (20 µg) probed with JunD antibody at a dilution of 1:1,000.

JunD antibody (pAb) tested by CUT&RUN
CUT&RUN was performed using 500,000 K562 nuclei and sequenced using 38 base-pair, paired-end reads on the Illumina NovaSeq. Data was collected from 46 million reads, and JunD data is shown for Chromosome 19.

Background

JunD (Jun D proto-oncogene) is a member of the JUN family and forms a component of the AP-1 transcription factor complex. JunD binds DNA as a dimer. Other AP-1 family members consist of c-Fos, Fra-1, Fra-2, FosB, c-Jun, and JunD.

Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for JunD antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with JunD antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting