RBPJ antibody (mAb)

RRID: AB_2614972
Clone: 1F1
Aliases: SUH, csl, AOS3, CBF1, KBF2, RBP-J, RBPJK, IGKJRB, RBPSUH, IGKJRB1
Catalog No: 61505 Format: 100 µg ¥88,000 Buy
Catalog No: 61506 Format: 10 µg ¥24,000 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2b
Purification:
Protein G Chromatography
Host:
Rat
Molecular Weight:
68 kDa
Reactivity:
Human, Mouse

Applications

ChIP Validated Immunofluorescence Validated Electrophoretic Mobility Shift Assay Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
ICC/IF: 0.5 µg/ml dilution

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP
EMSA

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This antibody was raised against a full-length recombinant protein corresponding to human RBPJ.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

RBPJ antibody (mAb) tested by ChIP.
Chromatin IP was performed using chromatin of an Epstein-Barr virus infected lymphoblastoid cell line ( 2 x 10^6 cell eqivalents per ChIP) and RBPJ antibody or the equivalent amount of IgG negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using primer pairs for a positive and negative control region. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG.

RBPJ antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with RBPJ antibody at a 0.5 µg/ml dilution.

Background

RBPJ (Recombination Signal Binding Protein For Immunoglobulin Kappa J Region) is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with RBPJ antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting