BRD9 antibody (pAb)
RRID: AB_2614970 |
Aliases: PRO9856, LAVS3040 |
Catalog No: 61537 | Format: 100 µl | Discontinued | |
Catalog No: 61938 | Format: 50 µl | Discontinued | |
Catalog No: 61538 | Format: 10 µl | Discontinued |
Applications
Application Notes
This product has been discontinued:
Please refer to our alternative products Catalog No. 65705 and Catalog No. 91413
Validated Applications:
ChIP: 5 - 10 µl per ChIP
ChIP-Seq: 5 - 10 µl each
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution
IHC (FFPE): 1:100 dilution
ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.
Immunogen
This antibody was raised against a peptide within the C-terminal region of human BRD9.
Buffer
Purified IgG in PBS, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Background
BRD9 (bromodomain containing 9) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as ‘readers’ of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6) that function in the regulation of transcriptional activation and chromatin remodeling. There are five isoforms of BRD9 that are produced by alternative splicing.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with BRD9 antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting