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STAT3 antibody (pAb)
| RRID: AB_2793718 |
| Aliases: APRF, HIES, ADMIO |
| Catalog No: 61645 | Format: 100 µl | ¥86,000 | Buy |
| Catalog No: 61646 | Format: 10 µl | ¥24,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
WB*: 1:1,000 - 1:2,500
*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This antibody was raised against a peptide within the N-terminal region of human STAT3.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
STAT3 antibody (pAb) tested by Western blot.
Detection of STAT3 by Western blot analysis. Nuclear extract of HepG2 cells (30 µg) probed with STAT3 antibody at a dilution of 1:2,000.
Background
STATs (signal transducers and activators of transcription) are a family of 7 transcription factors that form part of the JAK-STAT signaling cascade. This cascade is the basis of the signal transduction mechanism for many cytokine receptors. Once activated by phosphorylation by JAKs, STATs translocate to the nucleus. Accumulation of STATs in the nucleus is both rapid and tightly controlled. A number of factors regulate the JAK-STAT pathway including STAT dephosphorylation by phosphatases, altered nuclear import-export dynamics of STAT, and STAT gene activation antagonists such as SOCS (suppressors of cytokine signaling) and PIAS (Protein Inhibitors of Activated STATs).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
