Histone H3 antibody (mAb)

RRID: AB_2650522
Clone: MABI 0301
Catalog No: 39763 Format: 100 µg ¥88,000 Buy
Catalog No: 39064 Format: 50 µg ¥52,000 Buy

Request a quote for a bulk order Request Quote


Product Review Submit a Review


Antibody Type:
Monoclonal
Isotype:
IgG2b
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
17 kDa
Reactivity:
Human, Wide Range Predicted

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
ChIP-Seq: 4 µg per ChIP
ChIP: 5 - 10 µg per ChIP
ICC/IF: 1 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

For Histone H3, we also offer AbFlex® Histone H3 Recombinant Antibody (rAb). For details, see Catalog No. 91297.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-Seq, ChIP-qPCR
ICC/IF
WB
Proximal Ligation Assay (PLA)

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Histone H3 antibody (mAb) was raised against a peptide containing the N-terminus of histone H3.

Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

Histone H3 antibody (mAb) - 100 µg (Cat. No. 39763)

 
 
 

 

Histone H3 antibody (mAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from Farage human lymph-node derived B cells and 4 µg Histone H3 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 17.7 million sequence tags were mapped to identify Histone H3 binding sites.

Histone H3 antibody (mAb) tested by ChIP.
ChIP performed on Active Motif Ready-to-ChIP HeLa cell chromatin (Catalog No. 53015) using Histone H3 antibody (mAb). PCR was performed using primers specific for the promoter region of the human GAPDH gene.
Lane 1: ChIP using mouse IgG as a negative control.
Lane 2: ChIP using 10 µg of Histone H3 antibody (mAb).
Lane 3: Input DNA control.

Histone H3 antibody (mAb) tested by immunofluorescence.
HeLa cells stained with Histone H3 antibody (mAb) at a dilution of 1 µg/ml.

Histone H3 antibody (mAb) tested by Western blot.
HeLa nuclear extract (20 µg per lane) probed with Histone H3 antibody (mab) at a dilution of 2 µg/ml.
    Lane 1: Nuclear extract of untreated HeLa cells.
    Lane 2: Nuclear extract of HeLa cells treated with sodium butyrate.

Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.

Each histone contains two domains. First, a main globular domain (C-terminal) forming the core of the nucleosome is involved in histone-histone interactions and in binding to the DNA. Secondly, an N-terminal tail is subject to post-translational modifications. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 antibody (Clone MABI 0301).

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

This antibody is manufactured by MAB Institute, Inc.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting