ATF-2 phospho Thr71 antibody (mAb)
RRID: AB_2793467 |
Clone: 103C411 |
Aliases: HB16, CREB2, TREB7, CREB-2, CRE-BP1 |
Catalog No: 40975 | Format: 100 µg | Discontinued |
Applications
Application Notes
This product has been discontinued:
Applications Validated by Active Motif:
WB: 1 - 2 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
Immunogen
This ATF-2 phospho Thr71 antibody was raised against a synthetic peptide containing phosphorylated threonine at position 71 corresponding to human Phosphorylated-ATF-2.
Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Background
ATF-2 (activating transcription factor 2) transcription factor is a member of the leucine-zipper family of proteins and was previously known as cAMP responsive element binding protein 2 (or CREB2). ATF 2 is a transcriptional activator that binds the cAMP-responsive element (CRE) and interacts with c-Jun to stimulate CRE transcription. This protein is also capable of acetylating Histone H2B and Histone H4 in vitro as a histone acetyltransferase (HAT).
Positive Control
Active Motif's HeLa nuclear extract (TNF-α stimulated) (Catalog No. 40210) can be used as a positive control for ATF-2 phospho Thr71 antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting