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クロマチン修飾に関連する酵素の阻害効果など、修飾変化がグローバルに起こる場合、サンプル間の違いを同定することは非常に困難です。さらに、サンプル量の測定が不正確であったり、作業中の手技的なばらつきがあったりすると、サンプル間のデータにもばらつきが生じます。
このような場合、現在利用可能なバイオインフォマティクスに基づく標準化手法を、データセット間の標準化に適用することはできません。このため、サンプル量や手技による偏りやばらつきを克服するための唯一の信頼できる方法は、すべてのサンプルに既知の標準物質(spike-in)を添加する方法です。アクティブ・モティフは、ATAC-Seq、ChIP-Seq、CUT&RUN、CUT&Tagに利用可能なのspike-in標準化のための試薬を提供しています。
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