Recombinant Estrogen Receptor α protein

Aliases: ESR1, ESR, Era, ESRA, ESTRR, NR3A1
商品コード: 31119 Format: 4 µg ¥101,700 Buy
Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

Estrogen receptors (ER) are nuclear hormone receptors that mediate the actions of the endogenous steroid hormone, 17β-estradiol (E2). ER is synthesized as two protein forms, ERα and ERβ, which can bind to the estrogen receptor element (ERE) as homo- or heterodimers. Cell proliferation and differentiation triggered by ER activation has been heavily studied in bone-wasting and breast cancer treatments. Estrogen receptors are necessary for sexual development and reproductive function.

Application Notes

Recombinant ER is suitable for DNA and protein-protein interaction assays. 20-100 ng are sufficient for in vitro transcription assays and 100 ng are sufficient for protein-protein interaction studies. The molecular weight of the protein is ~64 kDa. This protein is not recommended for use in TransAM®. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

Protein Details

Recombinant ER is specific for the ER alpha subunit and was expressed from full length (accession number NM 000125) with an N-terminal His-Tag in a baculovirus system and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.