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Recombinant SETD2 (1392-2564) protein
| Aliases: HYPB; SET2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP |
| 商品コード: 31399 | Format: 20 µg | ¥99,000 | Buy |
| 商品コード: 31799 | Format: 1 mg | ¥788,500 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
SETD2 (SET Domain Containing 2) is a histone methyltransferase that methylates histone H3 at lysine 36. H3K36 methylation is associated with active chromatin. SETD2 also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA pol II. Recombinant SETD2 (1392-2564) includes amino acids 1392-2564 that contains the SET domain of SETD2 that catalyzes methylation of lysine residues.
Application Notes
Recombinant SETD2 (1392-2564) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
HMT Assay Conditions: 2 µg recombinant nucleosomes were incubated with SETD2 (1392-2564) in reaction buffer for 3 hr at RT. Western blot was used to detect reaction products (H3K36me2 Antibody, Cat# 39891). SETD2 (1392-2564) alone was used as negative control.
Protein Details
Recombinant SETD2 (1392-2564) (accession number NP_054878.5) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 134 kDa. The recombinant protein is >85% pure by SDS-PAGE.
Recombinant SETD2 (1392-2564) protein gel.
SETD2 (1392-2564) protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
MW: 134 kDa.
Purity: > 70%
HMT Assay for Recombinant SETD2 (1392-2564) protein activity. 2 µg recombinant nucleosomes were incubated with SETD2 (1392-2564) in reaction buffer for 3 hr at RT. Western blot was used to detect reaction products (H3K36me2 Antibody, Cat# 39891). SETD2 (1392-2564) alone was used as negative control.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
