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Recombinant BPTF / FALZ (2791-2911) protein
| Aliases: FAC1; NURF301 |
| 商品コード: 31447 | Format: 100 µg | ¥90,000 | Buy |
| 商品コード: 31847 | Format: 1 mg | ¥608,000 | Buy |
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Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
BPTF (Bromodomain PHD finger transcription factor) is a histone-binding component of NuRF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. BPTF protein contains a DNA-binding domain, a zinc finger motif and a bromodomain. Bromodomains recognize acetylated histone lysine residues and function as ‘readers’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. BPTF also specifically recognizes H3 tails trimethylated on lysine 4 (H3K4me3), which mark transcription start sites of virtually all active genes. It may also regulate transcription through direct binding to DNA or transcription factors.
Application Notes
Recombinant BPTF / FALZ (2791-2911) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Protein Details
Recombinant BPTF / FALZ (2791-2911) protein that includes amino acids 2791-2911 of human FALZ protein (accession number NP_872579.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with a molecular weight of 20.3 kDa. The purity of the protein is >90% by SDS-PAGE.
Recombinant BPTF / FALZ (2791-2911) protein gel.
BPTF / FALZ (2791-2911) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.
MW: 20.3 kDa
Purity: > 90%
HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity.
3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BPTF / FALZ (2791-2911) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.
HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity.
3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BPTF / FALZ (2791-2911) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
