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CAF-1 p60 antibody (mAb)
| RRID: AB_2793423 |
| Clone: 24 |
| Aliases: CAF1, MPP7, CAF-1, CAF1A, CAF1P60, CAF-IP60, MPHOSPH7 |
| 商品コード: 39995 | Format: 100 µg | ¥89,000 | Buy |
| 商品コード: 39996 | Format: 10 µg | ¥25,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
ICC/IF: 1:2,000 dilution
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
IP
ICC/IF
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
The CAF-1 p60 antobody was raised against full-length CAF-1 purified from human 293 cells.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
CAF-1 p60 antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with CAF-1 p60 antibody at a 1:2,000 dilution.
CAF-1 p60 antibody (mAb) tested by Western blot.
CAF-1 p60 (mAb) detection by Western blot. The analysis was performed using WT mouse testis and the CAF-1 p60 (mAb) at a 1 µg/ml dilution.
Background
CAF-1 p60 (Chromatin assembly factor-1 p60 subunit, CAF1B, CHAF1B) is a subunit of the CAF-1 complex that is required for the assembly of nucleosomes onto newly replicated DNA. Specifically, CAF-1 assists in the loading of the H3-H4 tetramer and may be involved in DNA damage repair. CAF-1 p60 is a WD-repeat containing protein related to HIRA.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
