ATRX antibody (pAb)

RRID: AB_2793487
Aliases: alpha thalassemia/mental retardation syndrome X-linked protein, X-linked helicase II, ATP-Dependent Helicase ATRX, Mental Retardation, X-Linked 52
商品コード: 61049 Format: 100 µl ¥89,000 Buy
商品コード: 61050 Format: 10 µl ¥25,000 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
300 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:1,000 - 1:5,000 dilution

Immunogen

This ATRX antibody was raised against a peptide within the C-terminus of human ATRX.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
ATRX antibody (pAb) tested by Western blot.

Western blot of ATRX antibody.
Nuclear extract of CCF-STGG1 cells (40 µg per lane) probed with ATRX antibody (pAb) at a dilution of 1:2,500.

Background

ATRX (alpha thalassemia/mental retardation syndrome X-linked protein, X-linked helicase II, XNP) is a transcriptional regulator and a member of the SWI/SNF family of chromatin remodeling proteins. It contains an SNF2 domain and helicase domain, and a PHD finger. ATRX undergoes cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in interphase gene regulation and chromosomal segregation in mitosis. Mutations of the ATRX gene are associated with an X-linked mental retardation syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting