Bcl6 antibody (mAb)

Application Notes

This antibody is also available as an AbFlex^® engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91287.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-Seq
IHC(P)
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Bcl6 antibody was raised against a full-length protein corresponding to human Bcl6.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Background

Bcl6 (B-cell lymphoma 6 protein, Bcl5, LAZ3, ZBTB27, ZNF51) is sequence-specific transcriptional repressor that is required for the formation of lymph node germinal centers (GC). Bcl6 is a member of the BTB-POZ family of transcriptional regulators and contains six Kruppel-type zinc fingers. The POZ domain in the amino terminus of Bcl6 is involved in the recruitment of corepressor proteins including NCoR, BCoR, SMRT, Mi-2/NURD and several HDACs. Bcl6 promotes proliferative expansion of B-cells in the germinal center through repression of genes regulating cell cycle and differentiation. Bcl6 also suppresses the DNA damage response, to prevent class-switch recombination being repaired. Unregulated expression of Bcl6 is involved in the development of many B-cell lymphomas.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

• ChIP = Chromatin Immunoprecipitation;
• ChIP = ChIP Sequencing;
• CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
• CUT&Tag = Cleavage Under Targets and Tagmentation;
• DB = Dot Blot;
• ELISA = Enzyme-linked Immunosorbent Assay;
• EMSA = Electrophoretic Mobility Shift Assay
• FC = Flow Cytometry;
• ICC = Immunocytochemistry;
• IF = Immunofluorescence;
• IHC = Immunohistochemistry;
• IP = Immunoprecipitation;
• MeDIP = Methyl-DNA Immunoprecipitation;
• NEU = Neutralizing;
• WB = Western Blotting