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NonO antibody (pAb)
| RRID: AB_2793567 |
| Aliases: Non-POU Domain Containing, Octamer-Binding, 54 KDa Nuclear RNA- And DNA-Binding Protein, P54, NMT55, NRB54, P54NRB |
| 商品コード: 61247 | Format: 100 µg | ¥89,000 | Buy |
| 商品コード: 61248 | Format: 10 µg | ¥25,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
IP: 5 µg per IP
WB: 0.5 - 2 µg/ml dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.
Immunogen
This NonO antibody was raised against a peptide corresponding to the C-terminus of human NonO.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
NonO antibody (pAb) tested by Immunoprecipitation.
5 µg of NonO antibody was used to immunoprecipitate NonO from 400 µg of HeLa whole cell extract (lane 2). 5 µg of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the NonO antibody at a dilution of 1 µg/ml.
NonO antibody (pAb) tested by Western blot.
Whole cell extract of HeLa cells (20 µg) probed with NonO antibody (1 µg/ml dilution).
Background
NonO (non-POU domain containing, octamer-binding, p54(nrb), NRB54, NMT55) is a DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. This gene also encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma.
Positive Control
Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for NonO antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
