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Recombinant MECP2 (80-166) protein
| Aliases: Methyl-CpG-binding protein 2, MeCp-2 Protein, MRX16, MRX79, RTT |
| 商品コード: 81474 | Format: 100 µg | ¥92,000 | Buy |
| 商品コード: 81574 | Format: 1 mg | ¥560,500 | Buy |
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Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
MECP2 (Methyl-CpG-binding protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5 methylcytosine (5mC).
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant MECP2 (80-166) protein that includes amino acids 80-166 of human MECP2 protein (accession number NP_004983.1) was expressed in E. coli cells with an N-terminal 6xHis tag. The molecular weight of the protein is 12.19 kDa.
References
Human
Recombinant MECP2 (80-166) protein gel.
12.5% SDS-PAGE gel stained with Coomassie Blue.
MW: 12.19 kDa
Purity: >95%
HTRF assay for Recombinant MECP2 (80-166) protein activity
1 μM substrate DNA (5’-Biotin-GCAGCCGG/5mC/GCGAATC-3’) were incubated with different concentrations of MECP2 (80-166) protein in HTRF binding buffer contain 50mM HEPES-NaOH pH 7.4,0.1%BSA for 1 hour, Then 10 μl detection reagents contain anti-His antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
