Recombinant Histone H1

商品コード: 81126 Format: 100 µg ¥28,000 Buy
商品コード: 81826 Format: 1 mg ¥107,500 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

Histone H1, also called as H1F0 (H1 Histone Family Member 0), is a replacement subtype, and replicationindependent histone, which is a member of the histone H1 family. It involves in basal repression of gene expression. H1 can act as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. Diseases associated with H1F0 include Retinal Cancer and Deafness, Autosomal Dominant.

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker- histone H1, with the DNA between the nucleosomes to form higher order chromatin structures. Histone H1 present at the interface between the nucleosome core and DNA entry/exit points.

Application Notes

Recombinant Histone H1 is suitable for use as substrates in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details

Full length Histone H1 (accession number NP_005309.1) was expressed in E. coli cells with no tag. The molecular weight of Histone H1 is 21 kDa.

 
Recombinant Histone H1 protein gel

Recombinant Histone H1 protein gel
12.5% SDS-PAGE Coomassie staining

Purity: >85%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.