Recombinant IGF2BP1 protein

Aliases: Insulin-like growth factor 2 mRNA-binding protein 1, ZBP1, VICKZ1, CRDBP,
商品コード: 81289 Format: 20 µg ¥92,000 Buy
商品コード: 81989 Format: 1 mg ¥788,500 Buy

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

IGF2BP1 (Insulin Like Growth Factor 2 MRNA Binding Protein 1), also called as ZBP1, VICKZ1 or CRDBP, is a member of the insulin-like growth factor 2 mRNA-binding protein family. The IGF2BP1 protein consists of two N-terminal RNA recognition domains (RRM1 and RRM2) and four C-terminal heterogeneous ribonucleoprotein (hnRNP) K homology (KH) domains (KH1–4). The mammalian IGF2 mRNA binding proteins (IGF2BPs) family encompasses three RNA-binding proteins (RBPs) controlling the cytoplasmic fate of mRNAs in development, somatic cells and human diseases. IGF2BPs are RNA-binding factors that recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript ‘caging’ into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Recent studies revealed that the association of IGF2BPs with target mRNAs, e.g. the MYC mRNA, is enhanced by the N6-methyladenosine (m6A) modification of target transcripts suggesting IGF2BPs as novel m6A-readers. N6- methyladenosine (m6A) is the most prevalent modification in eukaryotic RNAs. The biological importance of m6A relies on m6A readers, which control mRNA fate and function.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Full length human IGF2BP1 protein (accession number NP_006537.3) was expressed in a baculovirus system with an N-terminal FLAG-Tag. The molecular weight of the protein is 64.8 kDa.

 
Recombinant IGF2BP1 protein gel

Recombinant IGF2BP1 protein gel
10% SDS-PAGE with Coomassie blue staining

MW: 64.8 kDa

Purity: >78%

HTRF for IGF2BP1 activity

HTRF for IGF2BP1 activity
1 µM ACTB zipcode ssDNA (1212-1313) was incubated with different concentrations of IGF2BP1 protein in a 10 µl reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 µl anti-FLAG antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

HTRF for IGF2BP1 activity

HTRF for IGF2BP1 activity
1 µM ACTB zipcode ssDNA (1212-1313) was incubated with different concentrations of IGF2BP1 protein in a 10 µl reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 µl anti-FLAG antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of arrival.