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JunB antibody (pAb)
| RRID: AB_2615034 |
| Aliases: AP-1 |
| 商品コード: 39549 | Format: 200 µl | ¥89,000 | Buy |
| 商品コード: 39550 | Format: 10 µl | ¥25,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 1:500 - 1:2,500 dilution
Individual optimization may be required.
Immunogen
This JunB antibody was raised against a synthetic peptide corresponding to amino acids 28-42 of human JunB.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
JunB pAb tested by Western blot.
HeLa cell nuclear extract (20 µg per lane) was probed with JunB pAb at a dilution of 1:1,000.
Background
JunB (Jun B proto-oncogene) is a member of the Jun family and forms a component of the AP-1 transcription factor complex. JunB forms heterodimers and homodimers with other members of the Jun/Fos family. It is a hallmark of many lymphomas and stimulates proliferation. Other AP-1 family members consist of c-Fos, Fra-1, Fra-2, FosB, c-Jun, and JunD.
Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for JunB antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
